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HPLC

100 μg in 45 μl, 20 mM NaHCo3 (lyophilized) with high CaCl2 (0.1 mM). Lyophilized

with 45 μl of bi-distilled water

HPLC-purified mouse parvalbumin (recombinant, 100 ?g) produced in Escherichia Coli, after in vitro cDNA synthesis，This recombinant protein has been purified by HPLC and migrates as a single band of approximately 12 KDa

This protein can be used for immunoblots, and absorption experiments in immunehistochemistry. For intracellular injections in neurons with microelectrodes, we suggest using our other product AgPV10ic or AgPV100ic

After reconstitution freeze in small aliquots (e.g. 1 or 10 μg) and keep at -80°C. Avoid repeated freezing and thawing