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western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

Mouse monoclonal, IgG1

Conc. supernat. lyophil.

Lyophilized concentrated supernatant (no preservatives). 

with 200 μl / 500 μl or 1 ml bidistilled water

Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. 

The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C

Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.

Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. 

We recommend that the optimal dilutions be determined by titration experiments.

For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing. 

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 

2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 

3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 

4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493