少妇欧美成人精品日韩,欧美日韩一区二区三区精品视频不卡

您好，歡迎來(lái)到拜力生物網(wǎng)站！請(qǐng)登錄注冊(cè)

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時(shí)間：08:30-17:30

主營(yíng)產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測(cè)溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁(yè) 產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁(yè) > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

Mouse Embryonic Blasticidin and Zeocin-resistant (MMMbz) Immortalized Feeder Ce

訂購(gòu)信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Mouse Embryonic Blasticidin and Zeocin-resistant (MMMbz) Immortalized Feeder Ce

品牌：Abmgood

貨號(hào)：

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購(gòu)買(mǎi) 加入購(gòu)物車

Mouse Embryonic Blasticidin and Zeocin-resistant (MMMbz) Immortalized Feeder Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
Safety	BioSafety Level II
Organism	NMR1 Mouse
Source Organ	Embryo
Growth Properties	Adherent
Morphology	Fibroblast-like
Applications	For Research Use Only.
Picture
Description	Supporting cells, such as short-term cultures of fibroblasts, are used to cultivate human embryonic stem (ES) cells, however they are susceptible to senescence after a number of passages. The Mouse Embryonic Blasticidin and Zeocin-resistant (MMMbz) Immortalized Feeder Cells eliminates the problems associated with the use of primary cell lines. This cell line has a doubling time of 16 hours and may be used indefinitely to propagate human embryonic stem cells. It has the ability to facilitate the growth of hES H9 and hES H1. MMMbz cells are resistant to blasticidin and zeocin due to transgene expression allowing for selection of hES cells transfected with genes conferring resistance.
Quality Control	Selective growth with media containing blasticidin and zeocin to select for cells with transgene expression.
Propagation	Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: Bovine Calf Serum (available from gibco, SH30072) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. To freeze: suspend cells in PriGrow III (TM003) with 10% bovine calf serum and 10% sterile DMSO. To support the growth of hES cells: irradiate MMMbz cells with 6000 rads and seed at a density of 1.3x10⁵/cm² in propagation medium. The next day add hES in Knockout DMEM (Invitrogen), supplemented with 20% Knockout Serum Replacement (Invitrogen), 1mM L-glutamine (G275), 1% MEM non-essential amino acids (Invitrogen), 0.1mM 2-mercaptoethanol (CH045) and bFGF at 4 ng/ml (Z200015).
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.