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Human-Primary-Prostate-Stromal-Mesenchymal-Stem-Cells-(HPS-19I)

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Human-Primary-Prostate-Stromal-Mesenchymal-Stem-Cells-(HPS-19I)

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Human-Primary-Prostate-Stromal-Mesenchymal-Stem-Cells-(HPS-19I)

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Description	Mesenchymal stem cells are multipotent stromal cells with the ability to differentiate into other cell lineages such as cartilage, bone, and adipose tissues. Tissue-resident mesenchymal stem cells may have a role in repair, fibrosis, cardiovascular diseases, and tumor microenvironment. Studies have suggested that formation of myofibroblasts is associated with diseases such as cancer, fibrosis, and vessel disease. The Human Prostate Stromal Mesenchymal Stem Cells (HPS-19I) are isolated CD44⁺CD90⁺ cells from the ***** prostate gland which has the ability to differentiate into neurogenic, chondrogenic, and osteogenic cell lineages. In the presence of TGF-β1 the cells differentiate into myofibroblasts by inhibiting RUNX1 expression. The cells potentially are a source of reactive stroma fibroblasts. These cells are recommended in use for stem cell biology, stroma microenvironment, anticancer therapeutic and antifibrotic therapeutic studies.
Procedure Overview
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Prostate Gland
Growth Properties	Adherent
Morphology	Spindle-shape fibroblast-like
Image
Markers	CD44, CD90, CD13, CD29, CD73, CD105
Applications	For Research Use Only
Donor Gender	Male
Disease	Normal
Propagation	Use of PriCoat? T25 Flasks (G299) or . Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium to a final concentration of: 5% fetal bovine serum (TM999), 5% NuSerum, 0.5 μg/ml testosterone, 5 μg/ml insulin, and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C. Note: Density-dependent inhibition is exhibited at more than 70% confluency.
Quality Control	Flow cytometric analysis was performed to determine surface markers
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.