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SUNE2 Cells

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SUNE2 Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Human
Source Organ	Nasopharyngeal carcinoma
Disease	Stage III undifferentiated squamous cell carcinoma
Donor Age	38
Donor Gender	Female
Donor Ethnicity	Cantonese
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Description	The SUNE2 cell line is derived from a nasopharyngeal carcinoma (NPC) from the biopsy performed on a 38-year-old female Cantonese patient. This cell line has extremely low concentrations of Epstein-Barr virus (EBV) DNA in long-term culture and expressed low levels of latent membrane protein 1 (LMP1), latent membrane protein 2A (LMP2A), BamH1-A right frame 1 (BARF1), EBV-encoded RNA-1 (EBER1), and EBV-encoded RNA-2 (EBER2) in early passages. The SUNE2 cells also show a significantly stronger transforming ability than the control 5-8F cells in colony where it only requires 1 week for a single SUNE2 cell to form a colony of more than 200 cells. The SUNE2 cells are also able to form tumors in nude mice. As the proliferation of SUNE2 cells is significantly faster than other NPC cell lines, SUNE2 cell line is considered to be a new in vitro model which is highly valuable in research on the mechanisms underlying the occurrence and development of NPC.
Procedure Overview
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Propagation	The base medium for this cell line is Prigrow II medium available from abm(TM002). To make the completed growth medium, add the following components to the base medium: Fetal Bovine Serum (TM999) to final concentration of 10%, and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) Western blotting; 2) Immunostaining analysis using anti-keratin, anti-LMP2A, or anti-BZLF1; 3) Colony formation assay using statistical analysis
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.