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ASAN-PaCa Cells

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ASAN-PaCa Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Human
Source Organ	Pancreas
Disease	Intraductal papillary micinous neoplasm precursor lesion
Donor Age	61
Donor Gender	Female
Donor Ethnicity	Caucasian
Growth Properties	Adherent
Morphology	Polygonal
Population Doubling	Doubling time of 20 hours
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Description	ASAN-PaCa Cells are derived from tissue derived patient who underwent pancreatectomy. The cells were established to have chromosomal aberrations and exhibited high proliferation capacity. CK19 expression was observed indicating an epithelial phenotype. The cells may be used as a model of pancreatic cancer for further research in the field of pancreatic carcinogenesis.
Procedure Overview
Propagation	The base medium for this cell line is Prigrow II medium available from abm (TM002). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 20% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) Immunofluorescent staining confirmed CK19 expression; 2) qRT-PCR was preformed to analyze gene expression; 3) Karotyping was preformed
Reference	Heller, A., Angelova, A. L., Bauer, S., Grekova, S. P., Aprahamian, M., Rommelaere, J., . . . Giese, N. A. (2016). Establishment and Characterization of a Novel Cell Line, ASAN-PaCa, Derived From Human Adenocarcinoma Arising in Intraductal Papillary Mucinous Neoplasm of the Pancreas. Pancreas, 45(10), 1452-1460. doi:10.1097/mpa.0000000000000673
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.