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mThomson Factor Set Adenovirus (Mouse)

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mThomson Factor Set Adenovirus (Mouse)

商品詳情參考文獻相關資料

	Print Version
Vector	pAdeno
Promoter	CMV
Insert	mThomson Factor Set
Picture
Organism	Mouse
Accession #	Refer to individual adenovirus.
Alias	mOct4, mSox2, mNanog, mLin28, and GFP control virus
Protein Type	Wild Type
Functions	Co-expression of mOct4, mSox2, mNanog and mLin28 (Thomson set) in any somatic cell will lead to iPSC reprogramming. The Thomson set of transcription factors provides purified, high titer adenoviruses that offer the highest gene transduction efficiency for iPSC induction. The adenoviral vector will not integrated into host cell genome, avoiding possible mutations associated with vector insertion.
Gene Type	cDNA
Purification	By Ion-Exchange column.
Quality Control	By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming.
Titer	>1x10⁶pfu/mL
Storage	10 mM Tris-HCl, pH 8.0
Shipping	High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.
References	Induced pluripotent stem cell lines derived from human somatic cells. Yu et al. Science. 2007 Dec 21;318(5858):1917-20. Epub 2007 Nov 20. Human induced pluripotent stem cells free of vector and transgene sequences. Yu et al. Science. 2009 May 8;324(5928):797-801. Epub 2009 Mar 26. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al. Nature. 2009 Apr 9;458(7239):771-5. Epub 2009 Mar 1.