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Yamanaka Factor Set Adenovirus (Human)
品牌:Abmgood
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Yamanaka Factor Set Adenovirus (Human)

商品詳情 參考文獻(xiàn) 相關(guān)資料
Print Version
Vector pAdeno
Promoter CMV
Insert Yamanaka Factor Set
Picture
Organism Human
Accession # Refer to individual adenovirus.
Alias Oct4, Sox2, KLF4, Myc and GFP control virus.
Protein Type Wild Type
Functions Co-expression of Oct4, Sox2, KLF4 and Myc (Yamanaka set) in any somatic cell will lead to iPSC reprogramming. The Yamanaka set of transcription factors provides purified, high titer adenoviruses that offer the highest gene transduction efficiency for iPSC induction. The adenoviral vector will not integrated into host cell genome, avoiding possible mutations associated with vector insertion.
Gene Type cDNA
Purification By Ion-Exchange column.
Quality Control By sequencing and Western blot analysis of transgene expression in transduced cells. Functional test includes iPSC reprogramming.
Titer >1x106pfu/mL
Storage 10mM Tris-HCl, pH 8.0. 2.5% glycerol
Shipping High titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Notes For Research Use Only, not for therapeutic or diagnostic purposes.
References Induction of pluripotent stem cells from ***** human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72.

Induction of pluripotent stem cells from mouse embryonic and ***** fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.

Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji et al. Nature. 2009 Apr 9;458(7239):771-5. Epub 2009 Mar 1.
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