欧美日韩在线视频在线,少妇激情AV一区二区三区,别揉我奶子一区二区三区

您好，歡迎來到拜力生物網(wǎng)站！請(qǐng)登錄注冊(cè)

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時(shí)間：08:30-17:30

主營產(chǎn)品： ELISA試劑盒、抗體、補(bǔ)體血清、兔補(bǔ)體、干擾素、小鼠測(cè)溫儀、進(jìn)口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網(wǎng)站首頁產(chǎn)品中心代理品牌新聞資訊資源中心關(guān)于我們

所在位置：首頁 > 產(chǎn)品中心 > abmgood > abmgood產(chǎn)品

Immortalized-Human-Nucleus-Pulposus-Responder-Cells

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Immortalized-Human-Nucleus-Pulposus-Responder-Cells

品牌：Abmgood

貨號(hào)：

規(guī)格：

貨期：

應(yīng)用：

咨詢客服

立即購買加入購物車

Immortalized-Human-Nucleus-Pulposus-Responder-Cells

商品詳情參考文獻(xiàn) 相關(guān)資料

	Print Version
BioSafety Level	II
Organism	Homo sapiens
Source Organ	Disc Tissue
Growth Properties	Adherent
Morphology	Cobblestone, wave-like
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Transduction with retroviral particles expressing SV40LTAg (pBABE-hygro) and hTERT (pBMN-IRES-NEO)
Description	Immortalized Human Nucleus Pulposus Responder Cells were derived from healthy disc materials from patients and were immortalized via retroviral transduction carrying the SV40LTAg and hTERT genes. The cells retained its primary phenotype and express the SOX9, COL2A1 and COL1A1 markers. It has the ability to undergo chondrogenic differentiation, unlike the Immortalized Human Nucleus Pulposus Non-Responder Cells, available in abm, Cat. No. T0548. The Immortalized Human Nucleus Pulposus Responder Cells may be used to study the biology of the nucleus pulposus.
Procedure Overview
Image
Image
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow IV medium available in abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, 1% NEAA (Sigma, M7145), and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature Temperature: 37.0°C. Differentiation media: The base medium for differentiation is Prigrow IV medium available in abm, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, 1% ITS (TM052), 1% L-ascorbic acid 2-phosphate deoxycholate (Sigma), 1 ng/ml TGFβ3 (Cell Applications, RP1109-10), 1% NEAA (Sigma, M7145), and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature Temperature: 37.0°C.
Quality Control	1) 200 μg/ml G418 and 12 μg/ml hygromycin B for selection was used; 2) PCR utilized to detect chondrocyte markers; 3) Immortalization genes detected via immunoblot
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.