国产剧情av在线精品网站,日本欧美在线观看一区二区,色呦呦国内精品在线观看

您好，歡迎來到拜力生物網站！請登錄注冊

我的訂單 | 聯(lián)系我們 | 在線客服QQ：3012681483 | 客服電話：400-968-7988 | 工作時間：08:30-17:30

主營產品： ELISA試劑盒、抗體、補體血清、兔補體、干擾素、小鼠測溫儀、進口試劑、Quidel代理、Swant代理、PBL Assay Science代理

網站首頁產品中心代理品牌新聞資訊資源中心關于我們

所在位置：首頁 > 產品中心 > abmgood > abmgood產品

Immortalized-Mouse-Articular-Chondrocytes-(iMAC)

訂購信息

上海拜力生物科技公司

Tel:400-968-7988 021-33779008

Immortalized-Mouse-Articular-Chondrocytes-(iMAC)

品牌：Abmgood

貨號：

規(guī)格：

貨期：

應用：

咨詢客服

立即購買加入購物車

Immortalized-Mouse-Articular-Chondrocytes-(iMAC)

商品詳情參考文獻相關資料

	Print Version
BioSafety Level	II
Organism	CD1 Mouse Embryo
Source Organ	Tibial Plateau, Femoral Head, and Femoral Condyles
Growth Properties	Adherent
Morphology	Polygonal
Recommended Seeding Density	Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications	For Research Use Only
Immortalization Method	Serial passaging and infection with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
Description	The Immortalized Mouse Articular Chondrocytes are hygromycin-resistant, non-tumorigenic reversibly immortalized cells that retains both morphological and chondrogenic features. Specifically, it expresses chondrocyte markers such as Col 2a1 (type II collagen), aggrecan, Sox9, TNFα, IL-6, and COX2. In the presence of BMP2 (abm Cat. No. Z100085), the cells are able to produce mature chondroid matrix. These cells are unique, as the immortalization process may be reversed by infection with Cre-recombinase expressing adenoviruses (abm Cat. No. 000018A), thus reverting immortal cells back into primary cells. These cells are a valuable tool in chondrogenesis molecular mechanism, and cartilage tissue regeneration studies to identify biofactors promoting proliferation and differentiation of chondrogenic progenitors.
Procedure Overview
Image
Image
Propagation	Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control	1) RT-PCR, qPCR, sqPCR analysis for mouse gene of interest; 2) Col 1al immunohistochemical staining preformed; 3) Western blot used to detect SV40 large T antigen presence; 4) Alcian blue staining analysis for chondroid matrix production.
Disclaimer	1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.