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Concentration
1-10u /μl 
5'…GACGT↓C…3' 
3'…C↑TGCAG…5'

Reaction Conditions 
1X Buffer V5 
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg /ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.6), 50mM NaCl, 0.1mM EDTA, 200μg /ml BSA, 1mM DTT and 50% glycerol. Store at -20°C

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with AatII, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AatII for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A (Diluent) 
*High enzyme concentration may result in Star Activity. 
*Blocked by CpG methylation

Ordering Information

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AatII