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Concentration
1-2u/μl
5'…R↓CCGGY…3'
3'…YGGCC↑R…5'

Reaction Conditions 
1X Buffer V3 
50mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl₂, 100mM NaCl and 100μg/ml BSA. Incubate at 65°C.

Storage Buffer
10mM KH₂PO₄ (pH 7.5), 100mM NaCI, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.
*Enzyme is stable for up to 6 months if properly stored. It is recommended that the enzyme is kept in small aliquots to avoid repeated freeze-thaw cycles.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 2-fold overdigestion with Bse118I, 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of Bse118I for 16 hours at 65°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
Bse118I {Cfr10I}