国产精华液1区2区区别,国产欧美日韩一级在线,日本精品1区国产精品,亚洲国产成人精品综合

Concentration
1-4u/μl 
5'…G↓CGCGC…3' 
3'…CGCGC↑G…5'

Reaction Conditions 
1X Buffer V2 
10mM Tris-HCl (pH 7.5 at 30°C), 10mM MgCl₂, 50mM NaCl, and 100μg/ml BSA. Incubate at 50°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 4-fold overdigestion with BsePI, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 8u of BsePI for 16 hours at 50°C. 
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Downloads
Manual
BsePI