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Concentration
10-30u/μl 
5'…T↓CCGGA…3'
3'…AGGCC↑T…5'

Reaction Conditions 
1X Buffer Bsp13I
10mM Tris-HCl (pH7.6), 10mM MgCl2, 200mM KCl and 100μg/ml BSA. Incubate at 50°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 20-fold overdigestion with Bsp13I, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of Bsp13I for 16 hours at 50°C. 
Supplied with 10X Buffer Bsp13I, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by overlapping dam-methylation (G^mATC): TCCGGATC and GATCCGGA

Ordering Information

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Manual
Bsp13I {BspMII}