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Concentration
10-40u/μl 
5'…GRGCY↓C…3'
3'…C↑YCGRG…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with FriOI, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of FriOI for 16 hours at 37°C. 
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
Enzyme is stable for up to 6 months if properly stored. It is recommended that the enzyme is kept in small aliquots to avoid repeated freeze-thaw cycles.

Ordering Information

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Manual
FriOI {HgiJII}