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Concentration
3u/μl
5'…TGG↓CCA…3'
3'…ACC↑GGT…5'

Reaction Conditions 
Buffer V4
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Dilution
Viva Buffer A, 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 0.1mM EDTA, 1mM DTT, 200μg/ml BSA and 50% glycerol.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 3-fold overdigestion with Msp20I, 80% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of Msp20I for 16 hours at 37°C. 
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent) 
*Blocked by overlapping dcmˉmethylation (C^mCWGG): TGGCCAGG

Ordering Information

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Manual
Msp20I {BalI}