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ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit
品牌:Vivantis Technologies
貨號:QV7002
規(guī)格:150 reactions
貨期:
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Home ViPrimePLUS Real-Time PCR Kits Veterinary Infection qPCR/RT-qPCR Kits Other Veterinary ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit Description The ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Epizootic Hemorrhagic Disease Virus geno

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Home  ViPrimePLUS Real-Time PCR Kits  Veterinary Infection qPCR/RT-qPCR Kits  Other Veterinary  ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit

ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit

Description
The ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Epizootic Hemorrhagic Disease Virus genome in tissue samples such as spleen, lymph node, liver and lungs, and unclotted whole blood.
Epizootic Hemorrhagic Disease Virus (EHDV) is a single stranded RNA virus which is responsible for the epizootic hemorrhagic disease a.k.a. Ibaraki disease in deer, especially the white-tailed deer. EHD occurs in North American, Australia, Asia and Africa. There are 10 serogroups identified where each has different geographical distribution. Some of them can also affect cattle and most of other ruminants. The Ibaraki virus is the EHD serotype 2 discovered from the Japan, Korea and Taiwan outbreaks. The virus is very contagious and spreads very quickly in a herd of deer. There are 3 forms of syndromes observed in the infected deer; 1) Peracute form where high fever, anorexia, respiratory distress and edema of the head are seen, 2) Acute disease which is accompanied by haemorrhages in the skin, heart and gastrointestinal tract, and the 3) Chronic form where the recovered deer develop abnormal hooves, which paralyzes them, and ulceration in the rumen. No vaccines are available for EHDV, except for Ibaraki virus which is currently being used in Japan. 
ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit is intended for research use only.

Kit Component

  • EHDV Primers and Probe Mix (EHDV PPM)
  • Positive Control
  • Internal Extraction Control Primers and Probe Mix (IEC PPM)
  • Internal Extraction Control (IEC DNA)
  • Nuclease Free Water
  • Template Preparation Buffer

Product Specification

Technology Taqman probe-based real-time PCR assay
Type of Nucleic Acid Kit RNA
Kit Storage Shipped in room temperature, store at -20℃ on arrival
Sample Material Ruminant species samples (e.g. spleen, lymph node, liver and lungs, unclotted whole blood)
Gene Target NS1 gene
Detection Limit 10 to <100 copies per reaction
Sensitivity & Specificity Ct value 28±3 with probability of 95%
Controls Included ? Internal Extraction Control (IEC) as inhibition control
? Positive control
Channels ? FAM channels detect pathogen amplicons
? VIC/HEX channel detects IEC amplicons

Ordering Information

Catalog No Description Pack Size
QV7002 ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit 150 reactions

Download
Manual

ViPrimePLUS Epizootic Hemorrhagic Disease Virus RT-qPCR Kit

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